Waters Column Calculator . Obtaining Equivalent IEX Chromatographic Performance Through Automated An overview of the Columns Calculator to assist you in converting HPLC methods to UPLC methods. ANSWER: Use an online Columns Calculator (see ADDITIONAL INFORMATION to download) to determine the estimated back pressure based on your variables.
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Waters' Columns Calculator Scale your methods between di erent systems and across columns of di erent particle sizes and dimensions, while maintaining key separation performance. OBJECTIVE: Determine the volume of an empty column
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Source: www.waters.com Obtaining Equivalent IEX Chromatographic Performance Through Automated , Additionally, you are able to maintain the selectivity and resolution of a separation when running a method on a different system or on a column with different dimensions. The Columns Calculator enables you to scale a method by calculating operating parameters that give equivalent chromatographic performance
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Obtaining Equivalent IEX Chromatographic Performance Through Automated . OBJECTIVE: Determine the volume of an empty column PROCEDURE: Use Volume = pi x radius2 x Length pi = 3.14 r2 and Length should be converted to centimeters Diameter of column divided by 2 = radius.